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KMID : 0358420040470122424
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Korean Journal of Obstetrics and Gynecology
2004 Volume.47 No. 12 p.2424 ~ p.2429
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Insulin-like growth factor binding protein-3 protease profiles in sera of patients with uterine myoma before and after hysterectomy
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김정구/Kim JG
구승엽/지병철/서창석/김석현/최영민/문신용/Ku SY/Jee BC/Suh CS/Kim SH/Choi YM/Moon SY
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Abstract
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Objective: To investigate change in the activity of insulin-like growth factor binding protein-3 (IGFBP-3) protease and change in serum IGFBP-3 levels measurable by western ligand blot and immunoradiometric assay after hysterectomy, in patients with uterine myoma.
Methods: IGFBP-3 was analyzed by Western ligand blot (WLB) and immunoradiometric assay in sera obtained from patients (n=14) with uterine myoma undergoing hysterectomy, and IGFBP-3 protease activity were measured by protease assay using [125I] IGFBP-3 as substrate. Patients (n=13, control patients) undergoing tubal reanastomosis were served as controls.
Results: No significant differences in the preoperative and postoperative levels of serum IGFBP-3, and IGBPF-3 protease activity between patients with myoma and control patients were noted. In both group, serum IGFBP-3 level measured by WLB decreased significantly on 2nd day after operation and restored toward the preoperative level on 6th day after operation, while there was no significant change in postoperative serum IGFBP-3 levels measured by immunoradiometric assay. Serum IGFBP-3 protease activity increased significantly on 2nd day after operation and returned to the preoperative level on 6th day after operation in both group.
Conclusion: Circulating IGFBP-3 levels measurable by immunoradiometric assay does not change postoperatively and an increase in IGFBP-3 protease activity on 2nd day after operation result in a decrease in circulating IGFBP-3 levels measurable by WLB.
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KEYWORD
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